Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Furanodiene Induces Endoplasmic Reticulum Stress and Presents Antiproliferative Activities in Lung Cancer Cells

doi: 10.1155/2012/426521

Figure Lengend Snippet: FUR inhibition on colony formation in lung cancer cells. (a) Cells were treated with various concentrations of FUR for 24 h, after which morphological changes were observed using an AxioCam HRC CCD phase contrast microscope. Bar: 20 μ m. (b) Cells were treated with various concentrations of FUR for 24 h. After treatment, cells were suspended and reseeded into 6-well plates at a density of 200 cells per well (A549) or 1000 cells per well (95-D) and then fixed and stained with 4% PFA and crystal violet after one week. The representative images of colony formation assay were obtained. Bar: 2 mm.

Article Snippet: The human lung cancer cell lines A549 (ATCC, Rockville, MD, USA), NCI-H1299 (ATCC), and 95-D (The Cell Bank of Type Culture Collection of Chinese Academy of Sciences, Shanghai, China) were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (GIBCO BRL, Carlsbad, CA, USA), 100 U/mL penicillin, and 100 mg/mL streptomycin and were grown in an incubator with 5% CO 2 at 37°C.

Techniques: Inhibition, Microscopy, Staining, Colony Assay

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Furanodiene Induces Endoplasmic Reticulum Stress and Presents Antiproliferative Activities in Lung Cancer Cells

doi: 10.1155/2012/426521

Figure Lengend Snippet: FUR induces ER stress in lung cancer cells. (a) Effect of FUR on the mRNA levels of BIP and CHOP in A549 and 95-D cells. Cells were treated with different concentrations of FUR for 12 h, and mRNA levels were determined by RT-PCR. (b) Effect of FUR on the protein levels of BIP and CHOP in A549 and 95-D cells. Cells were treated with different concentrations of FUR for 12 h or 24 h, and protein levels were determined by Western blot. (c) Immunocytochemical staining was conducted to detect the expression of CHOP in nuclei. Cells were treated with 80 μ M FUR for 12 h and the cells were fixed and stained with anti-CHOP antibody (green) and Hoechst 33342 (blue). Bar: 10 μ m.

Article Snippet: The human lung cancer cell lines A549 (ATCC, Rockville, MD, USA), NCI-H1299 (ATCC), and 95-D (The Cell Bank of Type Culture Collection of Chinese Academy of Sciences, Shanghai, China) were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (GIBCO BRL, Carlsbad, CA, USA), 100 U/mL penicillin, and 100 mg/mL streptomycin and were grown in an incubator with 5% CO 2 at 37°C.

Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot, Staining, Expressing

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Furanodiene Induces Endoplasmic Reticulum Stress and Presents Antiproliferative Activities in Lung Cancer Cells

doi: 10.1155/2012/426521

Figure Lengend Snippet: CI values of FUR at concentrations applied in combination with DOX or TAX in different lung cancer cell lines.

Article Snippet: The human lung cancer cell lines A549 (ATCC, Rockville, MD, USA), NCI-H1299 (ATCC), and 95-D (The Cell Bank of Type Culture Collection of Chinese Academy of Sciences, Shanghai, China) were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (GIBCO BRL, Carlsbad, CA, USA), 100 U/mL penicillin, and 100 mg/mL streptomycin and were grown in an incubator with 5% CO 2 at 37°C.

Techniques:

Journal: BMC Cancer

Article Title: Identification of cancer cell-intrinsic biomarkers associated with tumor progression and characterization of SFTA3 as a tumor suppressor in lung adenocarcinomas

doi: 10.1186/s12885-024-13395-z

Figure Lengend Snippet: SFTA3 inhibits the proliferation and migration of lung cancer cells. A Relative expression levels of SFTA3 in three NSCLC cell lines. B Validation of SFTA3 knockdown and overexpression by RT-PCR. C Proliferation assay of H441 cells with the NC-vector or shSFTA3-vector; PC9 and A549 cells with the NC-vector or oeSFTA3-vector. D Colony formation assay of H441 cells with the NC-vector or shSFTA3-vector; PC9 and A549 cells with the NC-vector or oeSFTA3-vector. E Scratch wound healing assay of H441 cells with NC-vector or shSFTA3-vector; PC9 and A549 cells with NC-vector or oeSFTA3-vector; * P < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.001. All comparisons were made relative to the control group

Article Snippet: The human lung cancer cell lines H441, PC9, and A549 and the human embryonic kidney cell line 293 T were procured from the American Type Culture Collection (ATCC) (Manassas, VA, USA).

Techniques: Migration, Expressing, Biomarker Discovery, Knockdown, Over Expression, Reverse Transcription Polymerase Chain Reaction, Proliferation Assay, Plasmid Preparation, Colony Assay, Wound Healing Assay, Control